Composite

Part:BBa_K3258011:Design

Designed by: Alec Lourenco   Group: iGEM19_Stanford   (2019-10-16)


lacI-derived biosensor selection schema (positive selection) PREDCEL+


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 323
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 323
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 323
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Sequence synthesized via Twist.


Source

Source varies - it is a mixture of viral (T3 coliphage) and E. coli derived

References